{"id":323347,"date":"2021-12-09T10:30:00","date_gmt":"2021-12-09T09:30:00","guid":{"rendered":"https:\/\/innovationorigins.com\/?post_type=selected&p=323347"},"modified":"2021-12-09T10:30:00","modified_gmt":"2021-12-09T09:30:00","slug":"new-method-for-separating-molecules","status":"publish","type":"selected","link":"https:\/\/ioplus.nl\/archive\/en\/selected\/new-method-for-separating-molecules\/","title":{"rendered":"New method for separating molecules"},"content":{"rendered":"\n

University of Twente researcher Dr ir Saskia Lindhoud and PhD candidate ir J\u00e9r\u00e9 van Lente have achieved a breakthrough in their research on separating molecules. Their research was published this week in Wiley\u2019s international journal Small<\/a>, in the Rising Stars series, writes the University of Twente in a press release<\/a>. <\/p>\n\n\n\n

Dr ir Lindhoud: \u201cOur research process was inspired by how molecules are separated in biological systems. We have discovered a new method which enabled us to separate an enzyme from a complex mixture of proteins. The separated enzyme still had all its specific properties.\u201d<\/p>\n\n\n\n

Lindhoud explains: \u201cOur method to separate molecules could be useful for the recovery and recycling of valuable residual medicine molecules from waste water and it mayeven help in the recovery of certain chemical substances from waste water so that we can become less dependent on oil in the future. Unfortunately, we have not reached this point yet; we are still trying to understand this separation process in all its details. Our findings do, however, form a big stride in the right direction.\u201d<\/p>\n\n\n\n

Research process<\/h3>\n\n\n\n

Lindhoud and Van Lente simply used a packet of egg protein powder from the supermarket for their research. This powder contains a reasonable concentration of an enzyme known as \u2018lysozyme\u2019. Lindhoud: \u201cOur results show that we can indeed selectively extract lysozyme from this egg protein mixture. Furthermore, the lysozyme remains active. The method we use to separate the lysozyme molecules does not affect the structure of the enzyme and the enzyme is still able to break down its substrate. It is important that a separation procedure does not affect the properties biological molecules like enzymes .\u201d<\/p>\n\n\n\n

The separation of molecules<\/h3>\n\n\n\n

Lindhoud is inspired by separation processes in biological systems. Cells are, for example, extremely good at ensuring that the right molecules are at the right place to participate in biological processes. One way in which they realise this is by using compartments. In the classical view of the cell, these compartments are surrounded by a membrane and are known as organelles. These organelles float in cytosol, the intracellular fluid.<\/p>\n\n\n\n

However, it appears that the cytosol is very structured and also comprises compartments, which are called \u2018membraneless organelles\u2019. They look like droplets and form through water-in-water phase separation. One hypothesis is that these membraneless organelles are responsible for ensuring that the right molecules are at the right place at the right time. Lindhoud: \u201cIf we understand how membraneless organelles can separate so precisely, we may be able to use this for separating components in chemical processes. Many membraneless organelles form via interactions between positively and negatively charged groups on proteins and RNA.<\/p>\n\n\n\n

In our laboratory, we simulate this using positively and negatively-charged polymers. A polymer is a long molecule consisting of a long chain of equal parts known as monomers. The polymers we use have monomers that are either positively or negatively charged. If you mix solutions of these positively and negatively charged polymers, droplet-like structures are formed which have properties similar to those of membraneless organelles. Like membraneless organelles, our model droplets can selectively extract one protein, in our case, lysozyme, from a complex protein mixture.\u201d<\/p>\n\n\n\n

Also interesting: <\/strong><\/em>Production of basic molecules can be done more sustainably in Europe<\/a><\/p>\n","protected":false},"author":2084,"featured_media":323344,"template":"","meta":{"_acf_changed":false,"advgb_blocks_editor_width":"","advgb_blocks_columns_visual_guide":""},"categories":[42],"tags":[27331,61654,126080,11641,42797],"location":[6763],"internal_archives":[],"class_list":["post-323347","selected","type-selected","status-publish","has-post-thumbnail","hentry","category-sustainability-nl","tag-chemistry","tag-molecules","tag-recovery-of-substances","tag-recycling-en","tag-university-of-twente","location-netherlands"],"blocksy_meta":[],"acf":[],"featured_img":"https:\/\/ioplus.nl\/archive\/wp-content\/uploads\/2022\/04\/1VnVIQfg-molecules-ge1fba2f98_1920-1.jpg","coauthors":[],"author_meta":{"author_link":"https:\/\/ioplus.nl\/archive\/author\/mauro-mereu\/","display_name":"Mauro Mereu"},"relative_dates":{"created":"Posted 5 years ago","modified":"Updated 5 years ago"},"absolute_dates":{"created":"Posted on December 9, 2021","modified":"Updated on December 9, 2021"},"absolute_dates_time":{"created":"Posted on December 9, 2021 10:30 am","modified":"Updated on December 9, 2021 10:30 am"},"featured_img_caption":"\u00a9 Pixabay","tax_additional":{"category":{"linked":["Sustainability<\/a>"],"unlinked":["Sustainability<\/span>"],"slug":"category","name":"Categories"},"post_tag":{"linked":["chemistry<\/a>","molecules<\/a>","recovery of substances<\/a>","recycling<\/a>","University of Twente<\/a>"],"unlinked":["chemistry<\/span>","molecules<\/span>","recovery of substances<\/span>","recycling<\/span>","University of Twente<\/span>"],"slug":"post_tag","name":"Tags"},"language":{"linked":["EN<\/a>"],"unlinked":["EN<\/span>"],"slug":"language","name":"Tags"},"post_translations":{"linked":["pll_61b1caafd768b<\/a>"],"unlinked":["pll_61b1caafd768b<\/span>"],"slug":"post_translations","name":""},"location":{"linked":["Netherlands<\/a>"],"unlinked":["Netherlands<\/span>"],"slug":"location","name":"Locations"},"internal_archives":{"linked":[],"unlinked":[],"slug":"internal_archives","name":"Internal Archives"}},"series_order":"","_links":{"self":[{"href":"https:\/\/ioplus.nl\/archive\/wp-json\/wp\/v2\/selected\/323347","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ioplus.nl\/archive\/wp-json\/wp\/v2\/selected"}],"about":[{"href":"https:\/\/ioplus.nl\/archive\/wp-json\/wp\/v2\/types\/selected"}],"author":[{"embeddable":true,"href":"https:\/\/ioplus.nl\/archive\/wp-json\/wp\/v2\/users\/2084"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ioplus.nl\/archive\/wp-json\/wp\/v2\/media\/323344"}],"wp:attachment":[{"href":"https:\/\/ioplus.nl\/archive\/wp-json\/wp\/v2\/media?parent=323347"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ioplus.nl\/archive\/wp-json\/wp\/v2\/categories?post=323347"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ioplus.nl\/archive\/wp-json\/wp\/v2\/tags?post=323347"},{"taxonomy":"location","embeddable":true,"href":"https:\/\/ioplus.nl\/archive\/wp-json\/wp\/v2\/location?post=323347"},{"taxonomy":"internal_archives","embeddable":true,"href":"https:\/\/ioplus.nl\/archive\/wp-json\/wp\/v2\/internal_archives?post=323347"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}