{"id":448722,"date":"2023-06-07T11:00:00","date_gmt":"2023-06-07T09:00:00","guid":{"rendered":"https:\/\/innovationorigins.com\/?p=448722"},"modified":"2023-06-07T11:00:00","modified_gmt":"2023-06-07T09:00:00","slug":"revolutionary-cryonanosims-unveils-untapped-research-avenues","status":"publish","type":"post","link":"https:\/\/ioplus.nl\/archive\/en\/revolutionary-cryonanosims-unveils-untapped-research-avenues\/","title":{"rendered":"With the CryoNanoSIMS we see the world in a way we never could before"},"content":{"rendered":"\n<p>Scientists have developed the world&#8217;s first CryoNanoSIMS machine, enhancing nanoscale secondary ion mass spectrometry (NanoSIMS) analysis for observing biological tissue samples at sub-cellular spatial resolution, EPFL writes in<a href=\"https:\/\/actu.epfl.ch\/news\/epfl-scientists-unlock-new-horizons-for-cryogeni-2\/\"> a press release<\/a>. The instrument uses a sample preparation process pioneered by 2017 Nobel Prize winner Jacques Dubochet and offers new research opportunities.<\/p>\n\n\n\n<div class=\"io-block io-block__summary\"><ul><li>Scientists have developed the world&#8217;s first CryoNanoSIMS machine, which enhances nanoscale secondary ion mass spectrometry (NanoSIMS) analysis for observing biological tissue samples at sub-cellular spatial resolution.<\/li><li>This breakthrough in biological chemistry allows for the visualization of sub-cellular distribution of compounds, providing insights into bacterial infection, cancer treatment, plant growth, and environmental contaminants. <\/li><\/ul><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Overcoming Limitations of Traditional NanoSIMS Technology<\/h2>\n\n\n\n<p>NanoSIMS technology, introduced twenty years ago, revolutionised the imaging field by producing high-resolution images with a 100 nm resolution. However, traditional sample preparation methods had limitations \u2013 they caused distortion of tissue morphology and the loss of soluble compounds. The EPFL team, led by Prof. Anders Meibom, overcame these obstacles by developing a cryogenic process for sample preparation and modifying the NanoSIMS machine to accommodate cryogenic samples.<\/p>\n\n\n\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"This machine sees the world in a way we&#039;ve never seen it before #IOx #biology #nano #research #EPFL\" width=\"563\" height=\"1000\" src=\"https:\/\/www.youtube.com\/embed\/qWnegOFnK0M?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n\n\n\n<p>Meibom explained the challenges in adapting the instrument: &#8220;It was extremely difficult to turn an instrument that operates at room temperature into one that can analyse frozen tissue samples while keeping the sample cold and stable for hours and hours. But we succeeded, and can now obtain entirely new information.&#8221; To achieve this, the team collaborated with EPFL workshops and Swiss firms to leverage their mechanical engineering skills and to produce the required precision parts.<\/p>\n\n\n<div class=\"vlp-link-container vlp-layout-basic wp-block-visual-link-preview-link advgb-dyn-e8ba4f15\"><a href=\"https:\/\/ioplus.nl\/archive\/en\/roy-van-der-meel-develops-rna-nanotechnology-im-a-kind-of-postie-delivering-medicines\/\" class=\"vlp-link\" title=\"Roy van der Meel develops RNA nanotechnology. &#039;I&#039;m a kind of postie who delivers medicines&#039;\"><\/a><div class=\"vlp-layout-zone-side\"><div class=\"vlp-block-2 vlp-link-image\"><\/div><\/div><div class=\"vlp-layout-zone-main\"><div class=\"vlp-block-0 vlp-link-title\">Roy van der Meel develops RNA nanotechnology. &#8216;I&#8217;m a kind of postie who delivers medicines&#8217;<\/div><div class=\"vlp-block-1 vlp-link-summary\">When he explains at a birthday party what he researches, the scientist compares himself to a postal delivery driver. &#8220;When you order a parcel, the packaging should protect the contents. It should be a sturdy box that is easy to transport, and has the right address on it. It&#8217;s no different with nanoparticles. I also make packages, but so small that they are capable of delivering something into cells of the body.&#8221;<\/div><\/div><\/div>\n\n\n<h2 class=\"wp-block-heading\">Expanding the Field of Biological Chemistry<\/h2>\n\n\n\n<p>The CryoNanoSIMS machine is <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/37280616\/\">a significant breakthrough<\/a> in biological chemistry. It analyses the chemical and isotopic composition of vitrified tissue samples using the sample preparation process developed by Jacques Dubochet. Dubochet, the 2017 Nobel Prize winner in Chemistry and emeritus professor at the University of Lausanne, considers the CryoNanoSIMS instrument &#8220;an important expansion of the field of biological chemistry.&#8221;<\/p>\n\n\n\n<p>By visualising the exact sub-cellular distribution of compounds, CryoNanoSIMS has <a href=\"https:\/\/www.techexplorist.com\/cryo-imaging-reveals-layers-fuel-cell-catalysts\/59323\/\" data-type=\"URL\" data-id=\"https:\/\/www.techexplorist.com\/cryo-imaging-reveals-layers-fuel-cell-catalysts\/59323\/\">the potential <\/a>to shed light on essential processes underlying bacterial infection and cancer treatment, trace elements in plant tissue for plant growth and crop production, and environmental contaminants in soil and biofilms, all at sub-cellular spatial resolution.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Real-World Applications and Future Research<\/h2>\n\n\n\n<p>The study authors tested the CryoNanoSIMS method on Green Hydra samples, directly observing ammonium uptake and assimilation. This showcases the instrument&#8217;s capacity to provide unprecedented insights into biological processes. The next step for the researchers is to apply the CryoNanoSIMS method to corals, studying the mechanisms of symbiosis and the factors contributing to coral bleaching and death.<\/p>\n\n\n<div class=\"vlp-link-container vlp-layout-basic wp-block-visual-link-preview-link advgb-dyn-acd71d14\"><a href=\"https:\/\/ioplus.nl\/archive\/en\/researchers-from-twente-find-out-which-cyber-attacks-are-most-successful\/\" class=\"vlp-link\" title=\"Researchers from Twente find out which cyber attacks are most successful\"><\/a><div class=\"vlp-layout-zone-side\"><div class=\"vlp-block-2 vlp-link-image\"><\/div><\/div><div class=\"vlp-layout-zone-main\"><div class=\"vlp-block-0 vlp-link-title\">Researchers from Twente find out which cyber attacks are most successful<\/div><div class=\"vlp-block-1 vlp-link-summary\">Many people are online all day long. Apart from local outages, there \u00eds almost always Internet. How special is that? <\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"<p>Scientists have developed the world&#8217;s first CryoNanoSIMS machine, enhancing nanoscale secondary ion mass spectrometry (NanoSIMS) analysis for observing biological tissue samples at sub-cellular spatial resolution, EPFL writes in a press release. The instrument uses a sample preparation process pioneered by 2017 Nobel Prize winner Jacques Dubochet and offers new research opportunities. Overcoming Limitations of Traditional [&hellip;]<\/p>\n","protected":false},"author":2580,"featured_media":493867,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"advgb_blocks_editor_width":"","advgb_blocks_columns_visual_guide":"","footnotes":""},"categories":[76370,42],"tags":[39636,78655,76353,37973,36144,2558,4261],"location":[52892],"article_type":[36684],"serie":[],"archives":[],"internal_archives":[],"reboot-archive":[],"class_list":["post-448722","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-science","category-sustainability-nl","tag-epfl-en","tag-iron-mass","tag-laio","tag-nano-technology","tag-nanotechnologie-nl","tag-onderzoek","tag-research","location-switzerland","article_type-news"],"blocksy_meta":[],"acf":{"subtitle":"Scientists developed the world's first CryoNanoSIMS machine, which offers new research opportunities in areas such as bacterial infection, cancer treatment, and environmental contaminants. ","text_display_homepage":false},"author_meta":{"display_name":"Laio","author_link":"https:\/\/ioplus.nl\/archive\/author\/laio\/"},"featured_img":"https:\/\/ioplus.nl\/archive\/wp-content\/uploads\/2023\/06\/epfl.jpg","coauthors":[],"tax_additional":{"categories":{"linked":["<a href=\"https:\/\/ioplus.nl\/archive\/en\/category\/science\/\" class=\"advgb-post-tax-term\">Science<\/a>","<a href=\"https:\/\/ioplus.nl\/archive\/en\/category\/sustainability-nl\/\" class=\"advgb-post-tax-term\">Sustainability<\/a>"],"unlinked":["<span class=\"advgb-post-tax-term\">Science<\/span>","<span class=\"advgb-post-tax-term\">Sustainability<\/span>"]},"tags":{"linked":["<a href=\"https:\/\/ioplus.nl\/archive\/en\/category\/sustainability-nl\/\" class=\"advgb-post-tax-term\">EPFL<\/a>","<a href=\"https:\/\/ioplus.nl\/archive\/en\/category\/sustainability-nl\/\" class=\"advgb-post-tax-term\">iron mass<\/a>","<a href=\"https:\/\/ioplus.nl\/archive\/en\/category\/sustainability-nl\/\" class=\"advgb-post-tax-term\">Laio<\/a>","<a href=\"https:\/\/ioplus.nl\/archive\/en\/category\/sustainability-nl\/\" class=\"advgb-post-tax-term\">nano technology<\/a>","<a href=\"https:\/\/ioplus.nl\/archive\/en\/category\/sustainability-nl\/\" class=\"advgb-post-tax-term\">Nanotechnologie<\/a>","<a href=\"https:\/\/ioplus.nl\/archive\/en\/category\/sustainability-nl\/\" class=\"advgb-post-tax-term\">onderzoek<\/a>","<a href=\"https:\/\/ioplus.nl\/archive\/en\/category\/sustainability-nl\/\" class=\"advgb-post-tax-term\">research<\/a>"],"unlinked":["<span class=\"advgb-post-tax-term\">EPFL<\/span>","<span class=\"advgb-post-tax-term\">iron mass<\/span>","<span class=\"advgb-post-tax-term\">Laio<\/span>","<span class=\"advgb-post-tax-term\">nano technology<\/span>","<span class=\"advgb-post-tax-term\">Nanotechnologie<\/span>","<span class=\"advgb-post-tax-term\">onderzoek<\/span>","<span class=\"advgb-post-tax-term\">research<\/span>"]}},"comment_count":"0","relative_dates":{"created":"Posted 3 years ago","modified":"Updated 3 years ago"},"absolute_dates":{"created":"Posted on June 7, 2023","modified":"Updated on June 7, 2023"},"absolute_dates_time":{"created":"Posted on June 7, 2023 11:00 am","modified":"Updated on June 7, 2023 11:00 am"},"featured_img_caption":"","series_order":"","_links":{"self":[{"href":"https:\/\/ioplus.nl\/archive\/wp-json\/wp\/v2\/posts\/448722","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ioplus.nl\/archive\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ioplus.nl\/archive\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ioplus.nl\/archive\/wp-json\/wp\/v2\/users\/2580"}],"replies":[{"embeddable":true,"href":"https:\/\/ioplus.nl\/archive\/wp-json\/wp\/v2\/comments?post=448722"}],"version-history":[{"count":0,"href":"https:\/\/ioplus.nl\/archive\/wp-json\/wp\/v2\/posts\/448722\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ioplus.nl\/archive\/wp-json\/wp\/v2\/media\/493867"}],"wp:attachment":[{"href":"https:\/\/ioplus.nl\/archive\/wp-json\/wp\/v2\/media?parent=448722"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ioplus.nl\/archive\/wp-json\/wp\/v2\/categories?post=448722"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ioplus.nl\/archive\/wp-json\/wp\/v2\/tags?post=448722"},{"taxonomy":"location","embeddable":true,"href":"https:\/\/ioplus.nl\/archive\/wp-json\/wp\/v2\/location?post=448722"},{"taxonomy":"article_type","embeddable":true,"href":"https:\/\/ioplus.nl\/archive\/wp-json\/wp\/v2\/article_type?post=448722"},{"taxonomy":"serie","embeddable":true,"href":"https:\/\/ioplus.nl\/archive\/wp-json\/wp\/v2\/serie?post=448722"},{"taxonomy":"archives","embeddable":true,"href":"https:\/\/ioplus.nl\/archive\/wp-json\/wp\/v2\/archives?post=448722"},{"taxonomy":"internal_archives","embeddable":true,"href":"https:\/\/ioplus.nl\/archive\/wp-json\/wp\/v2\/internal_archives?post=448722"},{"taxonomy":"reboot-archive","embeddable":true,"href":"https:\/\/ioplus.nl\/archive\/wp-json\/wp\/v2\/reboot-archive?post=448722"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}